منابع مشابه
A simplified radioimmunoassay of plasma aldosterone.
The analytical evaluation is described of a [125I]-aldosterone radioimmunoassay method for measuring aldosterone in human plasma, which requires no chromatographic purification before quantification by radioimmunoassay but does require organic solvent extraction. Rabbit anti-aldosterone serum is used, generated against aldosterone-3-oxime coupled to thyroglobulin. The antibody demonstrates negl...
متن کاملSimplification of urinary aldosterone measurement by radioimmunoassay.
An anti-aldosterone serum has been used to measure urinary aldosterone concentration. A single stage of paper chromatography provides an extract of sufficient purity. Further purification by additional chromatography in a different solvent system only improves the purity by 3% +/- 8 (SD). Blanks are negligible and recovery of added aldosterone is 104% +/- 10 (SD).A simplified rapid screening as...
متن کاملQuantitation of plasma aldosterone by radioimmunoassay.
Aldosterone antibodies were produced by injecting sheep with the albumin conjugate of aldosterone. 18,21-dihemisuccinate. Conditions optimal for an accurate, reproducible assay with this antiserum were determined experimentally. Aldosterone is extracted from plasma and isolated by a single thin.layer chromatographic system before the immunoassay is performed. Normal plasma aldosterone in supine...
متن کاملNon-chromatographic radioimmunoassay procedure for urinary aldosterone.
We describe a sensitive, specific, and simple procedure for measuring aldosterone in human urine, which requires no chromatographic purification before quantification by radioimmunoassay but does include hydrolysis and extraction steps. Rabbit anti-aldosterone serum is sued, generated against aldosterone-18,21-dihemisuccinate coupled to human serum albumin. The antibody cross reacted little wit...
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ژورنال
عنوان ژورنال: Problems of Endocrinology
سال: 1995
ISSN: 2308-1430,0375-9660
DOI: 10.14341/probl11372